The Estimation of Fat
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چکیده
In the course of the nutrition investigations in this laboratoq m-e have occasion to make a great many estimations of fat in samples of portions of animals and in feeding stuffs. The usual Soxhlet extraction of the thoroughly dried sample with ether is the method that has been employed. It is understood that this method gives a determination of the crude fat or total ethersoluble material. By t.he Soxhlet extraction of dried blood we have been unable to find any ether-soluble substances. It has been very difficult to obtain concordant results with such material as brain, liver, and bran, and it has been found that sixteen hours extraction of these substances would not, remove all the ether-soluble mateyial. A re@xtraction frequently adds a very appreciable amount of crude fat. It is because of these difficultics t,hat we have investigated the methods proposed for accurate fat estimation in the hope that we could find a method that wuld give a true determination of the fat present, and at the s:tmc time permit application in a laboratory where a great, many tlrtcrminations must be made.
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